"There is growing need for a more stringent system to identify drug induced mitochondrial toxicity" explains Gareth."The aim of our work is to set up a real time drug screening system using high through put methodologies to identify drug induced mitochondrial dysfunction.To identify drug induced mitochondrial liabilities,cells are forced to rely on mitochondrial oxidative phosphorylation by culturing cells on a glucose free – galactose based media.Any perturbations in mitochondrial function or increase in cell death represents a potential mitochondrial toxic insult.The methods include mitochondrial respiratory chain complex activity assessment using a seahorse bio新利手机客户端science analyser and flow cytometry analysis of both early and late stage cell death.In addition to this we are currently mapping the mitochondrial proteome using label free quantitative proteomics,with the aim being able to identify proteomic changes that may be causative to the identified toxicity.This screening platform,followed by in depth proteomic analysis aims to provide a means to (i) identify mitochondrial toxins (ii) identify the proteins involved in these toxicological responses."
Many congratulations to Gareth on receiving this prize.
