Use Your Eyes: A simple test for E. Coli

波莉安娜阿什福德is a guest web writer for Analyst. She is currently a PhD student at the University of East Anglia, UK.

在林格氏液大肠杆菌的检测

在林格氏液大肠杆菌的检测

Sophisticated analytical tests producing a readout which can be interpreted by the naked eye, or by image editing software, are becoming increasingly popular. They offer a low cost alternative to tests requiring expensive equipment and highly trained analysts.Jürgen Popp并从同事Friedrich Schiller Universityand theInstitute of Photonic Technology在Jena,德国,已经开发了DNA一种新的检测方法,该方法提供了一种在肯定结果的情况下的显着的颜色变化。

静脉注射液在医院的细菌污染是现代医学显著的关注,所以Popp和他的团队选择的检测E. Coli在林格氏液作为第一个测试自己的分析。该技术使用表面改性可以通过杂交结合互补的靶DNA的磁珠。将银沉积试剂盒提供了视觉颜色变化:为肯定结果时,通过酶银沉积产生的银纳米颗粒,转动样品黑色。除了作为一种廉价的和方便的定性试验,定量结果可以通过分析在标准图像编辑软件的颜色变化的数字照片中找到。

读全文,请cick on the link below. The paper will be免费阅读的未来三周.

Convenient detection of E. coli in Ringer’s solution
Martha Schwarz, Susanne Pahlow, Thomas Bocklitz, Carolin Steinbrücker, Dana Cialla, Karina Weber and Jürgen Popp
分析人士, 2013, Advance Article
DOI:10.1039/C3AN01240A

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