Analyst Blog

Some wonderful covers in this latest issue ofAnalyst.The colourful outside front cover comes from雅各伯菲利克在Diamond Light Source Ltd.,UK,who together with colleagues from the UK have prepared a study that demonstrates the use of standard morphological image processing techniques to reduce the hyperspectral image data of samples,containing discrete particles or domains,to a single average spectrum per particle.

Morphological analysis of vibrational hyperspectral imaging data
Jacob Filik,Abigail V.Rutter,Josep Sulé-Suso and Gianfelice Cinque
Analyst,2012，137,5723-5729
DOI: 10.1039/C2AN35914F

The informative inside front cover comes from a team of scientists from Japan.Masaya Miyazakifrom the国家先进工业科学技术研究所新利手机客户端and colleagues has been imaging protein crystals deposited on a nanodroplet through the use of Raman spectroscopy.获得高质量的蛋白质晶体对于确定其结构非常重要。This is often the first step in novel drug design.

Three-dimensional Raman spectroscopic imaging of protein crystals deposited on a nanodroplet
Nitahara先生，Masatoshi Maeki,Hiroshi Yamaguchi,山下健一，宫崎骏和前田秀明
Analyst,2012，137,5730-5735
DOI: 10.1039/C2AN35942A

在封底上，我们展示了通过maldi成像质谱分析药物及其代谢物等小分子的研究。Kwang Pyo Kimat the Department of Molecular Biotechnology,建国大学,Seoul,South Korea

A new combination MALDI matrix for small molecule analysis: application to imaging mass spectrometry for drugs and metabolites
Selina Rahman Shanta,Tae Young Kim,Ji Hye Hong,Jeong Hwa LeeChan Young Shin,Kyun-Hwan Kim,Young Hwan Kim,Sang Kyung Kim and Kwang Pyo Kim
Analyst,2012，137,5757-5762
内政部：10.1039/c2an35782h

这些文件将free to read for 6 weeks.

We enter the world of forensics for the outside front cover for this most recent issue of Analyst.Frederic Zenhausernand colleagues from theUniversity of Arizona,USA,塑料微流控装置上的PCR原型报告。

Optimization of multiplexed PCR on an integrated microfluidic forensic platform for rapid DNA analysis
马修DEstes,Jianing Yang,Brett Duane,Stan Smith,Carla Brooks,Alan Nordquist和Frederic Zenhausern
Analyst,2012，137,5510-5519
DOI: 10.1039/C2AN35768B

From哈马那斯加法里和昆廷SHanley在英国诺丁汉特伦特大学,UK,我们的内封面。This paper reports on optically sectioned planar format assays detected using quantum dot (QD) reporters arrayed along the opticalZ-axis on mica or glass substrates to form volume encoded arrays.

Analysis of layered assays and volume microarrays in stratified media
Homanaz Ghafari和Quentin S.Hanley
Analyst,2012，137,5520-5526
DOI: 10.1039/C2AN35767D

这些文件将free to read for 6 weeks.

The latest issue ofAnalysthas now gone online,so take a look at the wonderful covers and the work that inspired them!

On the front cover we have an image fromEditorial BoardmemberBoris Mizaikoff,Christine Kranzand colleagues from theUniversity of Ulm.Their group designed a miniaturized cell stretching device for mechanically stimulating living cells at physiologically relevant levels applicable to a wide variety of adherent cell types.单击下面的链接阅读更多信息。

Combining atomic force-fluorescence microscopy with a stretching device for analyzing mechanotransduction processes in living cells
e.Hecht,P.Knittel,e.Felder,P.DietlB.Mizaikoff and C.Kranz
Analyst,2012，137,5208-5214
DOI:10.1039/c2an36001b

我们的内封面来自Pak Kin Wong,The University of Arizona,USA,世卫组织与同事一起报告了一种混合电动技术，可以在生理流体中操纵哺乳动物细胞。Cells can be adjusted for separation and focusing applications.单击下面了解更多信息。

Electrokinetic focusing and separation of mammalian cells in conductive biological fluids
Jian Gao,Reza Riahi,Mandy L.是的。Sin,Shufeng Zhang and Pak Kin Wong
Analyst,2012，137,5215-5221
DOI:10.1039/C2AN35707K

在封底上，我们展示了Sherif A.埃尔安全Laboratory,国家材料科学研究所新利手机客户端,Japan.Uniform,三维介孔ordered monoliths lead to tailored mesocaptor design of heavy metals.多捕获系统实现了光学分离，and detection of ultra-trace level of ions in drinking water.This captor would develop a simple technique for wastewater treatment and management.

Optical mesosensors for monitoring and removal of ultra-trace concentration of Zn(II) and Cu(II) ions from water
Sherif A.El-Safty,M.a.Shenashen,M.Ismael,M.哈里和Md.R.Awual
Analyst,2012，137,5278-5290
DOI:10.1039/C2AN35484E

All of these cover articles will befree to read for 6 weeks.

The latest issue ofAnalystis online with three cover articles for you to enjoy.

The very informative front cover comes from余长田和郑文忠from theNational Yunlin University of 新利手机客户端Science and Technology和Kaohsiung Medical University,respectively,and colleagues from Taiwan.In their study they proposed a new approach for the detection of proteins to assess the response of fibroblasts to a material surface.Biomaterials play an important role in regenerative medicine and drug delivery,therefore the interactions between biomaterial surfaces and cells are important to characterize cell proliferation,组织的分化和再生。

利用质谱分析系统评估透明质酸修饰表面诱导的细胞蛋白反应：CD36过度表达，CD44,CDK9,和PP2A
Ming-Hui Yang,Shiang-Bin Jong,Chi-Yu Lu,Yu-Fen Lin,Pei-Wen Chiang,Yu-Chang Tyan and Tze-Wen Chung
Analyst,2012，137,4921-4933
DOI:10.1039/c2an35368克

We head to Canada for our inside front cover.凯思琳MGough和Susan G.W.Kaminskyjfrom theUniversity of Manitobaand theUniversity of Saskatchewan,together with colleagues have been studying the filamentous fungiAspergillus.This fungi can can affect humans through their roles in biotechnology and recycling,and can also be responsible for disease.

What they have discovered is that Surface Enhanced Raman Scattering (SERS) can provide chemical information on materials that are in close contact with appropriate metal substrates,在这种情况下，纳米图案的金表面和金纳米粒子（AuNP）。They have created conditions for AuNP formation within and on the surface ofAspergillus nidulanshyphae in order to explore their potential for SERS analysis.

Proof-of-principle for SERS imaging ofAspergillus nidulans菌丝使用体内synthesis of gold nanoparticles
Martin A.PrusinkiewiczFatemeh Farazkhorasani,James J.Dynes,Jian Wang,凯思琳MGough and Susan G.W.Kaminskyj
Analyst,2012，137,4934-4942
DOI:10.1039/C2AN35620A

在本月刊的封底，我们展示了何卫东,和Xiao Linat theVanderbilt Institute of Nanoscale 新利手机客户端Science and Technology,and theInstitute of Physics,Chinese Academy of 新利手机客户端Scienceswho together with colleagues have studied the analytical expression of Coulombic interaction between a nanorod and a nanoparticle.

The evaluation of Coulombic interaction in the oriented-attachment growth of colloidal nanorods
Weidong He,Junhao Lin,Xiao Lin,Ning LuMing Zhou and Kelvin H.L.Zhang
Analyst,2012，137,4917-4920
DOI:10.1039/C2AN35950B

All of these papers will be免费使用6周.So why not click through and have a read.

The latest issue ofAnalystis online,有三篇封面文章供您欣赏。

我们令人惊叹的封面来自Professor Robin Turner和Professor Michael Bladesat the University of British Columbia,Vancouver,加拿大。他们的研究证实了拉曼光谱在非侵入性检测已知触发自噬条件下活细胞的生物学相关变化方面的作用。

The centre image is a differential interference contrast image of two MCF-7 cells,a human breast cancer cell line,after being starved of glutamine for two days.

The surrounding four images are chemical maps based on Raman microspectroscopy of the same two cells rendered from chemically-selective band intensities that indicate the spatial distributions of DNA and RNA (upper-left);RNA only (upper-right);phospholipid (lower-left) mapped relative to nucleic acid;and hydroxyapatite (lower-right),which is a common marker for breast cancers and strongly associated with malignancy.

In each image,the red intensity indicates the highest concentration,blue the lowest.  The advantage of this type of chemical imaging,according to the authors,is that it is non-destructive and label-free.

Raman microspectroscopy of live cells under autophagy-inducing conditions
斯坦尼斯拉夫O。Konorov,Mario A.Jardon,杰姆斯M皮雷Michael W.Blades and Robin F.B.Turner
Analyst ,2012，137,462-4668
DOI:10.1039/C2AN35477B

在前内盖上，Dr Michael Hipplerand colleagues from Sheffield University,UK,introduce a variant of cavity-enhanced Raman spectroscopy (CERS) in which diode laser radiation at 635 nm is coupled into an external linear optical cavity composed of two highly reflective mirrors.Using optical feedback stabilisation,build-up of circulating laser power by three orders of magnitude occurs.Strong Raman signals are collected in forward scattering geometry.

The authors say CERS has the potential to become a new standard method to monitor Raman active species,in particular important homonuclear gases like nitrogen,oxygen or hydrogen that can not be easily detected by alternative spectroscopic techniques.

Cavity-enhanced Raman spectroscopy with optical feedback cw diode lasers for gas phase analysis and spectroscopy
Robert Salter,Johnny Chu and Michael Hippler
Analyst ,2012，137,4669-4676
DOI:10.1039/C2AN35722D

The image on the back cover is fromMridul Kanti Mandal和Kenzo Hiraokaof University of Yamanashi,Chuo,日本and their co-workers.他们开发了一种对质谱法进行远程和直接取样的方法。

They say that the method is easy to operate and versatile allowing any biological specimen to be sampled away from the instrument in a minimally invasive manner.

Solid probe assisted nanoelectrospray ionization mass spectrometry for biological tissue diagnostics
伊杜尔·坎蒂·曼达尔先生，Kentaro Yoshimura,Subhrakanti Saha,Satoshi Ninomiya,Md.Obaidur Rahman,Zhan Yu,Lee Chuin Chen,Yasuo Shida,Sen Takeda,Hiroshi Nonami and Kenzo Hiraoka
Analyst ,2012，137,4658-4661
DOI:10.1039/C2AN36006C

All these cover articles will befree to accessuntil the end of October,and don't forget to also take a look at this issue's热门文章.

最新一期的《分析员》已经出版，and as usual there are some interesting cover images and articles for you to enjoy.

On the front cover is a Communication from丽贝卡霍普金斯and colleagues fromDSTL Porton Down,英国。They demonstrate spatially offset Raman spectroscopy (SORS) using 1064 nm excitation to detect chemicals through a physical barrier such as a container.这种激发波长克服了目标化学物质的荧光问题，whilst retaining the benefits of the SORS technique for through-barrier detection.The authors say these advantages have a wide range of applications in both civilian and military environments.

Communication: Short-wave infrared excited spatially offset Raman spectroscopy (SORS) for through-barrier detection
Rebecca J.Hopkins,Suzanne H.Pelfrey and Neil C.Shand
Analyst ,2012，137,4408-4410
DOI:10.1039/c2an35681c

The inside front cover features a Critical Review from圭多维尔贝克and colleagues from the University of North Texas.Their review concentrates on the apparatus and techniques associated with deposition processes,并覆盖了高温和热能量状态，允许物种完整着陆。

评述：软着陆制备质谱法
Guido Verbeck,威廉·霍夫曼和芭芭拉·沃尔顿
Analyst ,2012，137,4393-4407
DOI:10.1039/C2AN35550G

Both these cover articles will be免费使用6周,别忘了看看第19期的热门文章。

Our latest issue is now available,so do take a look.

Featured on the front cover,郑庆东from theFujian Institute of Research on the Structure of Matter,Fuzhou,China,and co-workers,report on the synthesis and characterization of two silylated BODIPY dyes and their use in dye-encapsulated silica nanoparticles with switchable emitting wavelengths for cellular imaging.In order to realise multiplexed imaging,they doped two different dyes into the same nanoparticle,which is able to emit light with different colors based on a change in excitation wavelengths.

Silylated BODIPY dyes and their use in dye-encapsulated silica nanoparticles with switchable emitting wavelengths for cellular imaging
薛华红Zhuyuan Wang,Jing Yang,Qingdong Zheng,沈飞宗Yu Sheng,德沁竹Changquan Tang and Yiping Cui
Analyst,2012，137,4140-4149
DOI:10.1039/c2an35389j

在前内盖上，Natalie Cannand colleagues fromQueen's University,金斯顿Canada present full-dimensional computational fluid dynamics (CFD) simulations for nano electrospray ionization (ESI) with various emitter designs as a step towards an integrated approach to emitter design and characterization.

Characterization of microstructured fibre emitters: in pursuit of improved nano electrospray ionization performance
辛云武，Richard D.Oleschuk and Natalie M.Cann
Analyst,2012，137,4150-4161
DOI:10.1039/C2AN35249D

Both these articles will befree to access6周。

别忘了看看这个问题中还有哪些热门文章。

Take a look at the latest issue ofAnalyst,with two great covers and host of HOT articles for you to enjoy.

Thefront coverfeatures a HOT paper from our Editorial Board Chair,Paul Bohn,and his post-docSean Branagan.Paul and Sean demonstrateelectrochemical imaging using enhanced optical transmission(EC-EOT) by applying it to several categories of electrochemical processes,using a spectral mapping technique that can be miniaturized.They say that in future applications,EOT提供的增强光谱信息可能有助于分离法拉第电化学信号和非法拉第电化学信号，以及在与带电界面相关的不同位置发生的空间分辨现象。

Plasmonic response of electrified metal–liquid interfaces during faradaic and non-faradaic reactions by enhanced optical transmission
Sean P.布拉纳根和保罗W。Bohn
Analyst,2012,137,932-39
DOI:10.1039/C2AN35488H

(more…)

在这个问题上Analystwe have two lovely covers for you to admire.On the outside we have an image fromSangmin Jeonand colleagues fromPohang University of 新利手机客户端Science and Technology,Korea.In their paper they report a new way of detecting沙门氏菌细菌，a common cause of food poisoning.This simple,快速的，and cost-effective method used magnetic (superparamagnetic) nanoparticles and TiO_二nanocrystals.

A facile and sensitive detection of pathogenic bacteria using magnetic nanoparticles and optical nanocrystal probes
Jinmyoung JooChangyong Yim,Donghoon Kwon,Jaejin Lee,Hwa Hui Shin,Hyung Joon Cha and Sangmin Jeon
Analyst,2012，137,3609
DOI:10.1039/c2an35369e

FromZhong-Ping Yao在香港理工大学,China,comes the inside front cover.Together with a team from around China,they have been analyzing biological tissue at the molecular level.他们已经能够直接电离和分析植物和动物组织质谱在环境条件下。

Direct ionization of biological tissue for mass spectrometric analysis
Bin Hu,Ying-Han Lai,Pui-Kin So,Huanwen Chen and Zhong-Ping Yao
Analyst,2012，137,三千六百一十三
DOI:10.1039/C2AN16223G

These cover articles will be免费使用6周,so click through and have a read.